Journal: Bioactive Materials
Article Title: Dermal fibroblast-derived extracellular matrix (ECM) synergizes with keratinocytes in promoting re-epithelization and scarless healing of skin wounds: Towards optimized skin tissue engineering
doi: 10.1016/j.bioactmat.2024.12.030
Figure Lengend Snippet: Biocompatibility of imDFs-ECM in vivo . (A) ECM-induced inflammation assessment in vivo . KM mice were subcutaneously injected with imDFs-ECM in 5 % GelMa (namely imDFs-ECM + GelMa in yellow ring) and observed at 8, 24, and 72 h after injection. Equal volume 5 % GelMa was set as a control. (B) Histological evaluation and IHC staining. The skin tissues from Φ1.0 cm centered on the injection sites were retrieved. The inflammatory cells were indicated by blue arrows in H & E staining ( a ). IHC staining was used to detect the expression of CD45, CD54, CD68, and CD206. Positive staining cells were indicated by red arrows ( b ) and the number of positive cells in a random field of view is quantified ( c ).
Article Snippet: After being blocked with 5 % BSA, the sections underwent immunostaining with primary antibodies against CD45 (1:100 dilution; Wanleibio; Cat# WL00922), CD54 (1:100 dilution; Wanleibio; Cat# WL02268), CD68 (1:100 dilution; Santa Cruz; Cat# sc-20060), CD206 (1:100 dilution; proteintect; Cat# 18704-1-AP), TGB-β1 (1:200 dilution; proteintect; Cat# 21898-1-AP) or COL1A1 (1:300 dilution; proteintect; Cat# 14695-1-AP).
Techniques: In Vivo, Injection, Control, Immunohistochemistry, Staining, Expressing